1,2-DCA Natural Attenuation Evaluation in Groundwater: Insight by Dual Isotope 13C/37Cl and Molecular Analysis Approach

Natural attenuation (NA) processes represent a valuable option in groundwater remediation. At heavily 1,2-dichloroethane (1,2-DCA) contaminated site, Compound-Specific Isotope Analysis (CSIA) combination with Biological Molecular Tools (BMTs) were implemented as rigorous characterization approach to evaluate the occurrence of Attenuation proximity source area. By use microcosm experiments, potential for natural and enhanced biodegradation under anaerobic conditions was documented, following dichloroelimination pathway. Enrichment factors −9.1‰ −11.3‰ obtained 13C while Geobacter spp. reductive dehalogenase genes (rdhs) identified main site-specific biomarkers. pilot scale, enrichments 13.5‰ 6.3‰ δ13C δ37Cl, respectively, high levels (rdh group VI) along dominance indicated significant conditions. using enrichment factors, degradation extents over approximately 70–80% estimated, highlighting relevant NA 1,2-DCA vicinity area at site. The proposed fine-tuned protocol, including CSIA BMTs, is proven be effective remediation strategy, properly assessing monitoring site scale.